Abstract
A detailed study has been performed on the antioxidant activity of the acetone and methanol extracts of the stem bark of the plant, Shorea roxburghii. The total phenolic content and antioxidant activity of the extracts were determined by DPPH, radical scavenging, ferric ion reducing power, hydroxyl radical, ABTS. radical scavenging and hydrogen peroxide scavenging activities. Reducing efficiency of the S. roxburghii towards silver nanoparticles has been evaluated using surface plasmon resonance and transmission electron microscope. Spherical shapes of particles with 4–50 nm have been reported. Formation of silver nanoparticles ascertains the role of the water soluble phenolic compounds present in S. roxburghii. Both acetone and methanol extracts of S. roxburghii stem bark was found to be a potent antioxidant. This work provides a scientific support for the high antioxidant activity of this plant and thus it may find potential applications in the treatment of the diseases caused by free radical. The extract of this plant could be used as a green reducing agent for the synthesis of Ag nanoparticles.
Highlights
There has been intense interest recently among the public and the media in the possibility that increased intake of dietary antioxidants may protect against chronic diseases, which include cancers, cardiovascular, and cerebrovascular diseases
Total phenolic content Total phenolic content of acetone and methanol extracts of stem bark of S.roxburghii was determined by the method of Singletone and Rossi, 1965. 10 mg of individual plant extract was dissolved in methanol to get the appropriate concentration (1 mg/mL). 1.0 mL of each extract in a test tube was mixed with 5.0 mL of distilled water. 1.0 mL of Folin-Ciocalteau reagent was added and mixed thoroughly. 3 min later, 3.0 mL of saturated sodium carbonate solution was added and the mixture was allowed to stand for 90 min in the dark
Total phenol content The amount of total phenols present in acetone and methanol extract of stem bark of S.roxburghii was determined from the regression equation (y = ax + b) of calibration curve of gallic acid standard solution and expressed in gallic acid equivalents (Figure 1)
Summary
There has been intense interest recently among the public and the media in the possibility that increased intake of dietary antioxidants may protect against chronic diseases, which include cancers, cardiovascular, and cerebrovascular diseases. Previous phytochemical study of the Shorea species revealed the presence of various stilbenoids (Tukiran et al 2005). Some of these stilbenoids which show interesting biological activities such as cytotoxic (Seo et al 1999 & Aminah et al 2002), antioxidant (Tanaka et al 2000; Saisin et al 2009), antiplatelet aggregation (Aburjai, 2000) and cyclooxygenase inhibitory activities (Li et al 2001). The aim of the present study is to explore the antioxidant potential of acetone and methanol extracts of stem bark of Shorea roxburghii. DPPH, ABTS, hydroxyl radical and hydrogen peroxide scavenging activities and ferric reducing power assays have been to evaluate the antioxidant activity of these extracts. The reducing ability of water extract towards Ag nanoparticles has been evaluated
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