Antioxidant Activity of the Chemical Constituents Isolated from the Roots of Albizia ferruginea (Guill. and Perr.) Benth. (Fabaceae)

Abstract

The present study was carried out to evaluate the antioxidant activity of chemical constituents isolated from <i>Albizia ferruginea</i> grapes, a plant of the Fabaceae family. The plant is used in traditional pharmacopoeia to treat dysentery, bronchial infections, infections from stings, gallbladder caused by fever and intestinal problems. Chemical study of the CH₂Cl₂-MeOH (1:1) extract led to the isolation of six compounds: (22E, 24S)-stigmasta-5,22,25-trien-3β-ol (1); 3,4,5,7-tetrahydroxyflavone (luteolin) (2); quercetin (3); kaempferol-7-O-β-D-glucoside (4); alphitolic acid (5); β-sitosteryl palmitate (6). The compounds (22E, 24S)-stigmasta-5,22,25-trien-3β-ol (1); 3,4,5,7-tetrahydroxyflavone (luteolin) (2); kaempferol-7-O-β-D-glucoside (4); alphitolic acid (5) and β-sitosteryl palmitate (6) were isolated from <i>Albizia ferruginea</i> for the first time. The structures of the isolated compounds were determined based on spectroscopic analysis and comparison of their spectral data with those in literature. The results of the antioxidant test revealed that at a dosage of 75 μg/mL the percentage weight of compound 3 was the highest, at dosages of 100 and 300 μg/mL, compound 2 was highest and at dosage of 150 μg/mL, compound 1 was the highest. The anti-radical activities of the compounds were significantly different (p<0.05) and is less than that compared to vitamin C. The result of the FRAP (ferric reducing antioxidant power) test showed that the roots are the part of the plant with the highest antioxidant capability.<div><br></div>