Antioxidant Activity of Styrylpyrones from Inonotus rheades (Agaricomycetes) Mycelium under Oxidative Stress in Thellungiella salsuginea Cell Suspension Culture.

Abstract

This study examined effects of the styrylpyrone fraction from mycelium of hymenochaetoid species, Inonotus rheades (Agaricomycetes), on reactive oxygen species inhibition in Thellungiella salsuginea cell culture under oxidative stress. We identified antioxidant effects of styrylpyrones from I. rheades mycelium on oxidative stress in T. salsuginea cell suspension culture using two models of oxidative stress (induced by hyperthermia or hydrogen peroxide). The results showed that the styrylpyrone fraction maintained a high level of cell viability under stress conditions. The effect of pure hispidin on the plant cell culture was insignificant during the development of oxidative damage caused by hydrogen peroxide and was lacking during hyperthermia-induced oxidative stress. Therefore, these results suggest that the protective effect of the styrylpyrone fraction on T. salsuginea cells can be exerted by individual compounds (which are part of the fraction) and their complex.