Abstract
Objective: To evaluate the antioxidant activity of stem bark of Thespesia populnea . Materials and Method: Antioxidant activity of T. populnea stem bark was studied in four in vitro / ex vivo models, viz., DPPH radical scavenging activity, superoxide scavenging activity, reducing power assay and lipid peroxidation. Results: Preliminary phytochemical screening showed the presence of high amount of phenolics, tannins and flavonoids. Subsequent quantification by Folin Ciocalteau method showed the presence of high amount of total phenolics (10.11 % w/w, calculated as gallic acid). Methanolic extract of stem bark of T. populnea showed a very good DPPH radical scavenging activity (EC 50 of 12.08 μg) and superoxide scavenging activity (EC 50 of 73.53 μg) in a dose dependent manner. The reduction ability of the extract, (Fe 3+ to Fe 2+ transformation) was found to increase with increasing concentrations where a maximum absorbance of 1.012 was obtained at a concentration of 300 μg. It also inhibited lipid peroxidation in a dose dependent manner with an EC 50 of 20.76 μg, which was comparable to α-tocopherol (EC 50 of 27.35 μg). Conclusion: The methanolic extract of the stem bark of T. populnea showed very good free radical scavenging activity, including inhibition of lipid peroxidation and reducing power.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
