Abstract

Natural products have the potential to be developed into new drugs for the treatment of various diseases. The aim of the present study was to screen the antioxidant activities of some common edible fruits, garden plants and medicinal plants indigenous to Taiwan. This was performed by assessing the activities of lipoxygenase, xanthine oxidase and tyrosinase following incubation with extracts from these plants. A further aim was to use HPLC-DAD and tyrosinase to chromatographically identify the antioxidative constituents obtained from an extract exhibiting strong antioxidative properties. The acetone extracts of 27 cultivated plant species from Taiwan were tested for antioxidant activities towards xanthine oxidase, tyrosinase and lipoxygenase using spectrophotometric assays. Koelreuteria henryi, Prunus campanulata, and Rhodiola rosea showed the highest xanthine oxidase inhibitory activities. Camellia sinensis, Rhodiola rosea, and Koelreuteria henryi exhibited good tyrosinase inhibitory activities and potent anti-lipoxygenase activities. As Koelreuteria henryi had notable significant inhibitory activities towards xanthine oxidase, tyrosinase, and lipoxygenase, it was further tested with tyrosinase and HPLC-DAD. The results from this part of the study revealed that the more powerful the antioxidant capability of the extracted component, the greater the decrease in peak height obtained after reacting with tyrosinase. Additional studies are warranted to further characterize the compounds responsible for the antioxidant properties of the examined extracts.

Highlights

  • The condition of in vivo “oxidative stress” is defined as elevated levels of free radicals or other reactive oxygen species (ROS) which can elicit either direct or indirect damage to the body

  • The extracts exhibiting greater than 50% of xanthine oxidase (XO) inhibitory activity were further investigated to determine the concentration that could inhibit 50% of enzyme activity (IC50)

  • All plant extracts were screened for tyrosinase inhibitory activity

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Summary

Introduction

The condition of in vivo “oxidative stress” is defined as elevated levels of free radicals or other reactive oxygen species (ROS) which can elicit either direct or indirect damage to the body. The extracts exhibiting greater than 50% of XO inhibitory activity were further investigated to determine the concentration that could inhibit 50% of enzyme activity (IC50). At 0.1 mg/mL, three plant extracts (Koelreuteria henryi, Prunus campanulata, and Rhodiola rosea) exhibited >50% inhibition of XO activity. All plant extracts were screened for tyrosinase inhibitory activity.

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