Antioxidant Activity of Selected Stilbenoid Derivatives in a Cellular Model System.

Treml Treml,Padrtová Padrtová,Granica Granica,Jankovská Jankovská,Paulíčková Paulíčková,Hošek Hošek,Leláková Leláková,Labuda Labuda,Havlík Havlík,Šmejkal Šmejkal

doi:10.3390/biom9090468

Abstract

The stilbenoids, a group of naturally occurring phenolic compounds, are found in a variety of plants, including some berries that are used as food or for medicinal purposes. They are known to be beneficial for human health as anti-inflammatory, chemopreventive, and antioxidative agents. We have investigated a group of 19 stilbenoid substances in vitro using a cellular model of THP-1 macrophage-like cells and pyocyanin-induced oxidative stress to evaluate their antioxidant or pro-oxidant properties. Then we have determined any effects that they might have on the expression of the enzymes catalase, glutathione peroxidase, and heme oxygenase-1, and their effects on the activation of Nrf2. The experimental results showed that these stilbenoids could affect the formation of reactive oxygen species in a cellular model, producing either an antioxidative or pro-oxidative effect, depending on the structure pinostilbene (2) worked as a pro-oxidant and also decreased expression of catalase in the cell culture. Piceatannol (4) had shown reactive oxygen species (ROS) scavenging activity, whereas isorhapontigenin (18) had a mild direct antioxidant effect and activated Nrf2-antioxidant response element (ARE) system and elevated expression of Nrf2 and catalase. Their effects shown on cells in vitro warrant their further study in vivo.

Highlights

Stilbenoids are a group of secondary plant metabolites belonging to a wide family of plant phenolics
Like the flavonoids represented by quercetin, the stilbenoids are quercetin was used for reference because it is a well‐known antioxidant, and at greater phenolics found at high concentrations in medicinal plants, vegetables, walnuts, and edible fruits, concentrations shows pro‐oxidant properties [6]
In combination stilbenoids are phenolics found at high concentrations in medicinal plants, vegetables, walnuts, and with anthocyanins, this compound is believed to be responsible for the beneficial phenomenon called edible fruits, such as grapes or berries [1]

Summary

Introduction

Stilbenoids are a group of secondary plant metabolites belonging to a wide family of plant phenolics. Among the stilbenoids chosen for these experiments (see Table 1) and one of the best known is trans-resveratrol (1). Biomolecules 2019, 9, 468; doi:10.3390/biom9090468 www.mdpi.com/journal/biomolecules the basal level and after stimulation with pyocyanin [5] after short‐ and long‐termed exposure to determine any possible antioxidative and pro‐oxidative effects, and compare its effects with quercetin [6]. These compounds that showed potential to interact with oxidative processes were further tested for their effects on the expression of enzymes involved in oxidative stress: catalase

Methods

Results

Discussion

Conclusion