Abstract
When lipids are exposed to heat, light and oxygen, it leads to oxidation. The addition of antioxidants is required to preserve colour, flavour and vitamin destruction. Present study was, therefore, planned to investigate pod coat of pigeon pea as possible sources of natural antioxidants and to assess their efficacy in stabilization of crude soybean oil during normal storage (28 days at 50°C). Study revealed that acetone pod coat extract of pigeon pea showed richness in total phenolics (17.72 mg/g), flavonoids (9.00 mg/g) and tannins (2.21 mg/g) while the extract of ethyl acetate was found enriched in tocopherols content (9.56 mg/g). The IC50 value of acetone extract was found to be lowest, exhibited potent antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric thiocyanate (FTC) methods. After adding synthetic and natural antioxidants in oil, Peroxide, p-Anisidine, Thiobarbituric acid value, Conjugated dienes, trienes and free fatty acids content were measured every 4 days. Acetone pod coat extract (2000ppm) of pigeon pea gave strong antioxidant efficacy in stabilization of crude soybean oil and hence could be recommended as natural antioxidants for food applications.The research explored the possibility of using pod coat of pigeon pea as imminent sources of green antioxidants and to evaluate their efficacy in stabilization of crude soybean oil.
Highlights
The use of natural extracts for medicinal purpose has taken prominent dimensions in the past few decades, largely due to the discovery that these contain primary metabolites and minerals and a great variety of secondary metabolites having antioxidant properties [1] [2]
Study revealed that acetone pod coat extract of pigeon pea showed richness in total phenolics (17.72 mg/g), flavonoids (9.00 mg/g) and tannins (2.21 mg/g) while the extract of ethyl acetate was found enriched in tocopherols content (9.56 mg/g)
Seeds of pigeon pea are traditionally recommended to treat piles and biliousness
Summary
The use of natural extracts for medicinal purpose has taken prominent dimensions in the past few decades, largely due to the discovery that these contain primary metabolites and minerals and a great variety of secondary metabolites having antioxidant properties [1] [2]. Inclusion of antioxidants is required to conserve colour and flavour along with prevention of vitamin destruction Synthetic antioxidants such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), tert-butyl hydroquinone (TBHQ) and propyl gallate (PG) are generally used to preserve fatty foods. Pigeon pea (Cajanus cajan L.) commonly known as arhar or red gram is a perennial legume of Fabaceae family. It is an important legume of semi-arid, tropical and sub tropical regions. Seeds of pigeon pea are traditionally recommended to treat piles and biliousness. The leaves of this pulse crop are used in the treatment of abdominal tumours, diabetes, wounds and sores [5]. This study is an attempt to investigate pod coat of pigeon pea as effective sources of green antioxidants and to investigate their efficacy in stabilization of soybean oil
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