Abstract
Antioxidant effect of several pineal derived molecules has been well documented. Here, the protective effects of 5-methoxytryptophol (5-MTOH) and 5-methoxyindol-3-acetic acid (5-MIAA) on hepatic cell membrane lipid peroxidation and cell membrane rigidity induced by FeCl3 plus ascorbic acid have been systemically investigated. The membrane fluidity was evaluated by fluorescence spectroscopy, malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) concentrations and carbonyl groups of protein were measured as the parameters of lipid and protein damage, respectively. Results showed that oxidative stress increased membrane rigidity, MDA and 4-HDA concentrations as well as carbonyl content in a concentration-dependent manner. 5-MTOH, but not 5-MIAA, significantly attenuated these oxidative indecies. In absence of oxidative stress, none of these methoxyindoleamines modified the content of MDA, 4-HDA or carbonylation. However 5-MIAA at its highest concentration slightly modified membrane fluidity. The results suggest that structural modification of C3 in the methoxyindoleamine, that is, the carboxyl group replaced by hydroxyl group in this site could improve the ability of 5-methoxyindoleamine derivatives to preserve membrane fluidity of cells which are under oxidative stress.
Highlights
Antioxidant effect of several pineal derived molecules has been well documented
Http://www.melatonin-research.net nervous system from oxidative stress. It plays a pivotal role in protection of hydroxyl radical (OH)-induced carcinogenesis and neurodegeneration [10, 11], and preserves nucleic acids, lipids, proteins, and membrane fluidity disturbance caused by oxidative stress [12, 13]
Similar to melatonin which was isolated from bovine pineal gland by Lerner et al in 1958 [21], several years later, the 5-MTOH and 5-methoxyindol-3acetic acid (5-MIAA) were found in pineal gland and their chemical structures were characterized by researchers [22]
Summary
The protective effects of 5-methoxytryptophol (5-MTOH) and 5-methoxyindol-3acetic acid (5-MIAA) on hepatic cell membrane lipid peroxidation and cell membrane rigidity induced by FeCl3 plus ascorbic acid have been systemically investigated. It plays a pivotal role in protection of hydroxyl radical (OH)-induced carcinogenesis and neurodegeneration [10, 11], and preserves nucleic acids, lipids, proteins, and membrane fluidity disturbance caused by oxidative stress [12, 13]. The antioxidant and free radical scavenging activity of additional two pineal gland derivatives, 5-MTOH and 5-MIAA, are investigated in the current study. These 5-methoxyindoleamines are the products of monoamine oxidase-B (MAO-B).
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