Antioxidant activity of peanut seed testa and its antioxidative component, ethyl protocatechuate

Abstract

The antioxidant activity of ethanolic extracts of peanut seed testa (EEPST) and its antioxidative component, ethyl protocatechuate (EP), was examined. It was found that EEPST and EP showed a dose-dependent activity on the inhibition of liposome peroxidation. EEPST and EP in the range of 50–500mg/l were effective in protecting protein against oxidative damage. EEPST and EP at 100mg/l showed 92.6% and 84.6% scavenging effect, respectively, on α,α-diphenyl-β-picrylhydrazyl radical, indicating that they act as a primary antioxidant. In addition, EEPST and EP, at a dose of 200mg/l, showed 70.6% and 67.7% scavenging effect, respectively, on the hydroxyl radical. EEPST also exhibited a metal-binding ability, while EP did not. The inhibitory effect of EEPST on linoleic peroxidation correlated with their polyphenolic content. These results suggest that the antioxidant mechanism, for both EEPST and EP, could possibly be due to their scavenging effect on free radical and hydroxyl radical. In addition, its metal binding ability may contribute to antioxidant activity of EEPST.