Abstract
The purpose of this study was to determine, for the first time, antioxidant activities of seven peptides (P1–P7) derived from hydrolysis of oat proteins in a cellular model. In the oxygen radical absorbance capacity (ORAC) assay, it was found that P2 had the highest radical scavenging activity (0.67 ± 0.02 µM Trolox equivalent (TE)/µM peptide) followed by P5, P3, P6, P4, P1, and P7 whose activities were between 0.14–0.61 µM TE/µM). In the hepatic HepG2 cells, none of the peptides was cytotoxic at 20–300 µM. In addition to having the highest ORAC value, P2 was also the most protective (29% increase in cell viability) against 2,2′-azobis(2-methylpropionamidine) dihydrochloride -induced oxidative stress. P1, P6, and P7 protected at a lesser extent, with an 8%–21% increase viability of cells. The protection of cells was attributed to several factors including reduced production of intracellular reactive oxygen species, increased cellular glutathione, and increased activities of three main endogenous antioxidant enzymes.
Highlights
Health benefits associated with the consumption of grains are linked to the presence of phytochemicals such as polyphenols and to their content of fibers and micronutrients [1]
The mechanism of protection can be through the reduction of blood cholesterol/glucose, or the prevention of oxidative damage to biomolecules [1,2,3]
Benefits of consuming oat products have mainly been attributed to the presence of dietary fibers, phenolic acids and a unique group of amide derivatives known as avenanthramides [6]
Summary
The mechanism of protection can be through the reduction of blood cholesterol/glucose, or the prevention of oxidative damage to biomolecules [1,2,3]. Many studies have focused on the potential of chemicals in grains to prevent or attenuate oxidative stress. It was found, for example, in men with coronary artery disease, that the supplementation of their diets with whole grains (brown rice and barley) resulted in lower oxidation of plasma lipids [1]. Other studies found that oats consumed in the form of oatmeal, oat gum, or oat bran reduced blood low-density lipoproteins cholesterol by 2–23% in both healthy and hypercholesterolemic humans [3,4]. Benefits of consuming oat products have mainly been attributed to the presence of dietary fibers, phenolic acids and a unique group of amide derivatives known as avenanthramides [6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
