Antioxidant activity of <i>Vaccinium axillare</i> Nakai fruits during oxidative stress <i>in vivo</i>

Abstract

Intensity of free radical oxidation processes in vivo (model of induced oxidative stress) was studied after the probe introduction of Vaccinium axillare Nakai fruit extract. Material and methods. Four groups (n = 40) of white male CBA mice weighing 20–25 g were included in the experiment: 1 – intact control; 2–0.9 % sodium chloride solution was administered per os for 10 days in a dose of 10 ml/kg/day; 3 – group “cisplatin” (animals received 0.9 % sodium chloride solution similarly to group 2, on the fifth day of the experiment cisplatin was administered once by intraperitoneal injection at a dose of 7.5 mg/kg); 4 – group “cisplatin + blueberries” (mice received per os extract of Blueberry axillary fruits at a dose of 10 ml/kg/day for 10 days, on the fifth day of the experiment cisplatin was administered once by intraperitoneal injection at a dose of 7.5 mg/kg). Antioxidant activity of Blueberry axillary was studied by chemiluminescence. Results and discussion. Analysis of kinetic parameters of mouse kidney homogenate chemiluminescence showed that oxidative stress develops in animals after a single intraperitoneal injection of cisplatin, the extract of Blueberry axillary fruit decreases its severity. Conclusions. Bilberry fruit extract (Vaccinium axillare Nakai) has pronounced antioxidant properties and may be important in the treatment and prevention of diseases associated with oxidative stress.