Antioxidant Activity of <i>p</i>-Hidroxy-<i>m</i>-Methoxy Chalcone and its Combination with Doxorubicin: <i>In Vitro </i>and <i>In Silico</i> Study

Abstract

Chalcone has a variety of interesting biological activities, including as an antioxidant and anticancer. Antioxidants are molecules that can retard or prevent the oxidation process or inhibit the formation of free radicals. p-Hydroxy-m-Methoxy Chalcone (pHmMC) is a chalcone derivative reported has anticancer activity both in used single and in combination treatment with Doxorubicin (DOX) in breast cancer cell lines. DOX is one of the chemotherapy agents widely used in cancer treatment but the medicine has side effects of cardiotoxicity. This effect is generally associated with free radical formation. This study aims to find out the activity of pHmMC as an antioxidant both in used single and in combination treatment with DOX by in vitro and to explore the potential of pHmMC as an antioxidant by in silico. Activity tests as antioxidants were measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) test. Ascorbic acid (Vitamin C) was used as a positive control. Antioxidant activity was calculated as the value of 50% Inhibition Concentration (IC50). In silico study was carried out by molecular docking using Protein Ligand ANT System (PLANTS) software with peroxiredoxin 5 [1HD2] as the target. The results showed that the IC50 values of pHmMC, DOX, and Vitamin C were 11.9; 21.6 and 3.3 μ/mL respectively. The combination of pHmMC-DOX has a higher antioxidant activity than single pHmMC or single DOX. The docking molecular showed that there were similarities amino acids involved in the interaction between PRDX5[1HD2]-pHmMC and PRDX5[1HD2]-DOX. This research indicated that pHmMC and pHmMC-DOX are potentially developed as an antioxidant.