Antioxidant activity of l-adrenaline: A structure–activity insight

Abstract

l-Adrenaline belongs to a group of the compounds known as catecholamines, which play an important role in the regulation of physiological process in living organisms. The antioxidant activity and antioxidant mechanism of l-adrenaline was clarified using various in vitro antioxidant assays including 1,1-diphenyl-2-picryl-hydrazyl (DPPH ), 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), N, N-dimethyl- p-phenylenediamine (DMPD + ), and superoxide anion radicals (O 2 − ) scavenging activity, hydrogen peroxide (H 2O 2), total antioxidant activity, ferric ions (Fe 3+) and cupric ions (Cu 2+) reducing ability, ferrous ions (Fe 2+) chelating activity. l-Adrenaline inhibited 74.2% lipid peroxidation of a linoleic acid emulsion at 30 μg/mL concentration. On the other hand, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), α-tocopherol and trolox displayed 83.3, 82.1, 68.1 and 81.3% inhibition on the peroxidation of linoleic acid emulsion at the same concentration, respectively. BHA, BHT, α-tocopherol and trolox were used as reference antioxidants and radical scavenger compounds. Moreover, this study will bring an innovation for further studies related to antioxidant properties of l-adrenaline. According to present study, l-adrenaline had effective in vitro antioxidant and radical scavenging activity.