Antioxidant Activity of Green Tea Extract and Myricetin

Abstract

Free radicals are the major cause of oxidative stress, which can lead to inflammatory diseases, ischemic diseases, hemochromatosis, emphysema, and other illnesses. Antioxidant intake is essential for keeping the body's oxidative processes in balance. Green tea containt a natural antioxidant that is commonly consumed and readily available. Myricetin is a pure component of green tea. This study aims was to determine the antioxidant activity of green tea extract (GTE) compared with myricetin (MYR). Antioxidant activity properties were determined by 2,2-diphenyl-1 picrylhydrazyl (DPPH), 2,2 ′ -Azinobis(3-Ethylbenzthiazoline-6-Sulfonate) (ABTS), hydrogen peroxide (H2O2), NO scavenging, and ferric reducing antioxidant power (FRAP). The analysis results showed that the IC50 of GTE (24.00 µg/mL) was higher than MYR (4.68 µg/mL) in DPPH scavenging activity. The highest concentration of FRAP activity of GTE and MYR were 595.78±19.35 and 530.67±10.97 μM Fe (II)/µg. GTE also had a higher IC 50 value for ABTS reducing activity than MYR, at 25.16 µg/mL versus 16.78 µg/mL. According to H 2 O 2 scavenging activity, MYR has higher antioxidant activity (133.32 µg/mL) than GTE (137.31 µg/mL). Meanwhile, the NO scavenging assay results show that MYR (19.70 µg/mL) has a higher IC 50 value than GTE (7.10 µg/mL). In conclusion, GTE has potential antioxidant activity like its particular compound, MYR.