Antioxidant Activity of Extracts from Different Polar Solvents of Flavonoid Extract of Aurea helianthus Flower

Abstract

Objective:To study the in vitro antioxidant capacity of different polar solvent extracts of flavonoids from Aurea helianthus flower,and to investigate the correlation between flavonoids content and antioxidant activity. Methods:The ethanol extracts of Aurea helianthus flower were extracted by liquid-liquid extraction with different polar solvents. The flavonoids from Aurea helianthus flower were obtained:petroleum ether phase,ethyl acetate phase,n-butanol phase and aqueous phase extract. The total flavonoids in different polar solvent extracts were determined. The DPPH free radical scavenging ability,hydroxyl radical scavenging ability,reducing ability,superoxide anion ability and antioxidant capacity of the extracts were studied. The relationship between antioxidant activity and flavonoid content were analyzed. Results:The results showed that solvent extracts of flavonoids with different polarities from Aurea helianthus flower had antioxidant activities,and the antioxidant activities of ethyl acetate phase was the strongest,followed by n-butanol phase,ethanol extract,petroleum ether phase,aqueous phase. In the range of the mass concentration of the extract from 0.20 to 1.00 mg/mL,the antioxidant activity increased correspondingly with the increase of the mass concentration. At the mass concentration of extract was 1.00 mg/mL,the antioxidant activity of the ethyl acetate and n-butanol phase extracts was close to VC. There was a good correlation between flavonoid content and antioxidant activity in each extract(p<0.05).Conclusion:The flavonoids of Aurea helianthus flower have high antioxidant capacity in vitro,and the ethyl acetate phase and n-butanol phase extract can be used as the research focus of separating active ingredients.