Antioxidant Activity of Extract and a New Isolated Dioxaspiran Derivative of Tripleurospermum disciforme.

Abstract

ABSTRACTAntioxidants are important substances with the ability to protect the body from damage caused by free radical–induced oxidative stress. A variety of free-radical scavenging antioxidants exist within the body, many of which are derived from dietary sources such as fruits, vegetables, and teas. In this study, various extracts of Tripleurospermum disciforme. (C. A. Mey) Schultz Bip. (Compositae) with different solvents and a new isolated compound from its chloroform extract were tested by linoleic acid peroxidation for antioxidant activity using 1,3-diethyl-2-thiobarbituric acid as reagent. The chloroform extract was found to be the most active one (IC50 = 10.75 µg/ml), comparable to α-tocopherol (IC50 = 14.75 µg/ml) as positive control. The isolated dioxaspiran derivative showed an IC50 value of 185.50 µg/ml with the same condition. Since this is higher than the IC50 value of the crude chloroform extract, other effective compounds may be responsible for such a difference.