Abstract
Antioxidant activity test for the extract of ethyl acetate nutmeg stem bark (Myristica fragrans Houtt) was carried out using 2,2-diphenyl-1picrylhydrazil (DPPH). The test results of antioxidant activity of ethyl acetate nutmeg stem bark extract with DPPH method at a concentration of 25 ppm, 50 ppm and 100 ppm obtained IC50 value = 68.14 ppm with IC50 value of vitamin C (as positive control) 3.657 ppm. The results of column chromatographic separation of ethyl acetate nutmeg stem bark extract obtained 5 combined fractions namely MFEK 1, MFEK 2, MFEK 3, MFEK 4 and MFEK 5. The test results of the fraction antioxidant activity in a good category were the MFEK 1 fraction. The MFEK 2 fraction to MFEK 5 showed an antioxidant activity lower than compared to the ethyl acetate extract. This shows that the ethyl acetate nutmeg stem bark extract has a better inhibitory activity, because there are several active compounds contained in the extract which can inhibit free radicals, so the IC50 value of the extract is better than the fractions.
Highlights
Antioxidant activity was one of the targets of the study in this research that functioned as a natural ingredient in medicines
NO inhibition activity was shown by neolignan compounds from the chloroform extract [4] and methanol extract [5], whereas the methanol extract of pala mace contains neolignan compounds which show anticancer activity [6]
Antioxidant test of ethyl acetate extract of nutmeg stem bark and Vitamin C Ethyl acetate extract of nutmeg stem bark at concentration of 25 ppm 250 μL, concentration of 50 ppm 500 μL and concentration of 100 ppm 1000 μL, each added with DPPH solution of 0.4 mM as 1 mL and the volume was adjusted to 5 mL with methanol and container covered with aluminum foil
Summary
Antioxidant activity was one of the targets of the study in this research that functioned as a natural ingredient in medicines. Several studies on secondary metabolites in nutmeg leaves have been reported, among others Helen et al, 2012 [7] reported that nutmeg leaves oil contains 20 types of compound components with the main composition namely β-pinene 22.69%, and β-tujen 13,93% showed antimicrobial and anticancer activity. Phytochemical test of methanol nutmeg leaves extract have been reported to contain alkaloid compounds, flavonoids, terpenoids and tannins, while ethyl acetate extracts contain flavonoids and have antifungal activity against Candida albicans [8]. Antioxidant activity of ethyl acetate extract of nutmeg (Myristica fragrans Houtt) stem bark (Ilham Maulana, Binawati Ginting, Nurdin and Saiful Fakri) oxidative damage to biological macromolecules due to oxidation of various free radicals or commonly called Reactive Oxygen Species (ROS), where the main character of antioxidant compounds is their ability to capture and stabilize free radicals [18]. Nutmeg stem bark samples were taken from Paya Peulumat, East Labuhan Haji sub-district, South Aceh Regency
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