Abstract
Strawberry is a member of the rose family (Rosaceae, subfamily Rosoideae, tribe Potentilleae) in the genus Fragaria. The cultivated varieties of commercial strawberries usually were designated as Fragaria ananassa. Root, leaf, flower and fruit have the healing properties. The strawberry leaves extract is used for blood cleaning, for treatment of oral inflammation, diarrhea, various gastro-intestinal inflammation, and hemorrhoids, as well as a diuretic. So far, many positive biological effects of strawberries (anticancer, antioxidant and anticoagulant effect) have been proven. The purpose of this study was to evaluate the antioxidant potential of ethanolic extract from cultivated strawberries (Fragariae folium, varieties Senga Sengana) by using different antioxidant assays (DPPH, FRAP, FIC, H2O2 and TBA-MDA). Ethanolic extract from strawberry leaves was obtained by reflux extraction at the boiling temperature. Total phenols and total flavonoids content was determined spectrophotometrically by the method of Folin-Ciocalteu and by method with AlCl3, respectively. In the extract was determined high content of total phenols, while the total flavonoid content is much lower. The concentrations of extract required to neutralize 50% of the initial concentration of DPPH radicals (EC50) after 20 minutes incubation and immediately after adding DPPH radical solution were 7,91 and 19,46 ?gcm-3, respectively. Extract was achieved the maximum iron ions chelating ability (67.89%) at a concentration of 2 mgcm-3. Inhibition of lipid peroxidation of 70% was achieved by extract concentration of 0.03125 mgcm-3, while the maximum neutralization of H2O2 (30.47%) was achieved by extract concentration of 0.5 mgcm-3. FRAP value of the investigated extract is 284.51 mgFe/g of dry extract. Presented results of the antioxidant activity show that the obtained extract from the cultivated strawberry leaves is a potential source of natural antioxidants.
Highlights
U epruvetu je dodato po 0,1 cm3 ekstrakta i 3 cm3 FRAP reagensa i nakon inkubacije od 30 minuta na 37 °C izmerena je apsorbanca na 593 nm
Ova jedinjenja su najverovatnije velikim delom odgovorna za visok stepen inhibicije lipidne peroksidacije etanolnim ekstraktom lista jagode
The purpose of this study was to evaluate the antioxidant potential of ethanolic extract from cultivated strawberries (Fragariae folium, varieties Senga Sengana) by using different antioxidant assays (DPPH, FRAP, FIC, H2O2 and tiobarbiturnom kiselinom (TBA)-MDA)
Summary
Lekovito bilje se koristi u ishrani kao začin, za pripremu čajnih mešavina, kao i u medicinske svrhe za izradu fitopreparata. Bioaktivni proizvodi iz biljnih sirovina, poznati kao prirodni izolati ili prirodne biohemikalije, imaju sve veću primenu u proizvodnji lekova, kozmetičkih i prehrambenih proizvoda ili kao prekursori u sintezi novih proizvoda sa specifiičnim bioaktivnim svojstvima [2]. Gajene (baštenske) sorte komercijalnih jagoda obično su označene kao Fragaria ananassa [3]. List jagode sadrži flavonoide (kvercetin i rutin), tanine, etarska ulja, vitamin C i fenolna jedinjenja, u većoj meri nego u plodu pa se zato upotrebljava za pravljenje čajeva [7,8]. Najveći broj ispitivanja odnosi se na ekstrakte iz ploda jagode, dok su ekstrakti iz lista gajenih jagoda, kao izvor bioaktivnih jedinjenja sa potencijalnim korisnim biološkim dejstvima manje ispitivani [8,9,10]. Cilj ovog rada je određivanje antioksidativne aktivnosti etanolnog ekstrakta lista gajene jagode sorte Zenga zengana, primenom različitih antioksidatvinih testova
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