Abstract
Ethanol extracts of Guazuma ulmifolia L. and Citrus hystrix Dc., have a strong antioxidant activity. The purpose of this research is to determine the antioxidant activity of ethanol extracts combination of Guazuma ulmifolia L. (jati belanda leaf) and Citrus hystrix Dc (jeruk purut leaf). Determination of antioxidant activity was done by DPPH method (1,1-diphenyl-2picrylhydrazil) using UV-Vis spectrophotometer with maximum wavelength 516.5 nm. The antioxidant activity of jeruk purut leaf is smaller than jati belanda leaf (IC50 48.70 mg/mL for jeruk purut leaf, IC50 35.92 mg/mL for jati belanda leaf). Antioxidant activity of the two extracts combination was lower than the antioxidants of each extract (without combination). The low antioxidant activity of both extract combination due to the antagonist activity as the interaction of the content of each extract so that the extract combination showing a weaker antioxidant activity. At the concentration of each different comparison of each extract combination volume shown no difference with P-value > 0.05 (P-value: 0.99).
Highlights
Free radicals are relatively unstable molecules with the outermost orbits of atoms that have one or more unpaired electrons [1]
This study aims to determine the antioxidant activity of the combination of both plants because of the high antioxidant activity
The method used to test the antioxidant activity of ethanol extract combination of jeruk purut leaf and jati belanda leaf is DPPH method (2,2diphenyl-1-picrylhydrazyl)
Summary
Free radicals are relatively unstable molecules with the outermost orbits of atoms that have one or more unpaired electrons [1]. Antioxidant is a compound that have an important role in maintaining health because it can capture free radical molecules thereby inhibiting oxidative reactions in the body which cause some various diseases [4]. The body must obtain sufficient amount of antioxidants Antioxidant Activity of Ethanol Extract Combinations of Jeruk Purut Leaf (Citrus hystrix Dc.) and Jati Belanda Leaf (Guazuma ulmifolia L.). Jati belanda leaf have antioxidant effects and phytochemical screening shows that it contains flavonoid compounds [7]. Jeruk purut leaf has exogenous antioxidant effect that can be used as an alternative to inhibiting the aging process (antiaging) because it contains citronellal, a powerful antioxidant. Phytochemical screening shows the jeruk purut leaf contain phenol, flavonoids, and terpenoids [8]. This study aims to determine the antioxidant activity of the combination of both plants because of the high antioxidant activity
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