Abstract
Chelidonium majus, from Papaveraceae family, is a rich source of different antioxidants with a range of medicinal activities including antispasmodic and diuretic properties. In this study, antioxidant potential of extracts from leaves during different phenological stages was measured by ferric-reducing power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Factors affecting antioxidant activity, i.e., total phenols, flavonoids, anthocyanin and carotenoids, were then investigated. Soluble sugar and total protein contents of samples were also determined. According to the results, maximum DPPH radical scavenging activity was 408/88 ± 24/83 g/g DW at growing stage, and the FRAP value reached maximum during fruiting stage (1.75 ± 0.04 mg/g FW). The leaves of flowering stage contained the most content of total phenol (17.8 ± 1.59 mg/g DW), flavonoid (69.7 ± 0.86 mg/g DW), anthocyanin (0.233 ± mg/g DW) and soluble sugar (0.338 ± 0.009 mg/g DW). However, the highest value for carotenoid (2.083 mg/g DW) and protein (0.27 ± 0.034 mg/g DW) was found at the vegetative stage.
Highlights
Production of chemically active molecules including reactive oxygen species (ROS) is a general response to stressful conditions that living organisms may encounter every day
The collected leaves samples of various stages were shadow dried, ground into powder and kept at 4 °C until each measurement, and some fresh samples were kept frozen for the Ferric-reducing antioxidant power (FRAP) test as recommended by the reference used [25]
The highest value was observed at flowering stage (17.8 ± 1.59 mg/g DW)
Summary
Production of chemically active molecules including reactive oxygen species (ROS) is a general response to stressful conditions that living organisms may encounter every day. The present research was designed aiming to determine the antioxidant activity and amount of total protein and soluble sugars in extracts of plant leaves during various stages of growth. Total phenol, total flavonoids, anthocyanins, carotenoids, proteins and sugars were measured in the different extracts from the plant parts.
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