Abstract
A new version of potentiometric evaluation of antioxidant activity in biological liquids, food, drinks, and so forth, based on the mediator system combined with the free radicals generation has been proposed. A radical initiator, 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), and K3[Fe(CN)6]/K4[Fe(CN)6] as a mediator system were used. Interactions of the mediator system with radicals, radicals with antioxidants, and erythrocytes have been investigated. The correlation coefficient between the data obtained in the presence and the absence of AAPH equals 0.98. In addition, the possibility to determine a free radical generation rate by using the mediator system has been demonstrated.
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