Abstract
Miconia is one of the largest genus of the Melastomataceae, with approximately 1,000 species. Studies aiming to describe the diverse biological activities of the Miconia species have shown promising results, such as analgesic, antimicrobial and trypanocidal properties. M. albicans leaves were dried, powdered and extracted to afford chloroformic and methanolic extracts. Total phenolic contents in the methanolic extract were determined according to modified Folin-Ciocalteu method. The antioxidant activity was measured using AAPH and DPPH radical assays. Chemical analysis was performed with the n-butanol fraction of the methanolic extract and the chloroformic extract, using different chromatographic techniques (CC, HPLC). The structural elucidation of compounds was performed using 500 MHz NMR and HPLC methods. The methanolic extract showed a high level of total phenolic contents; the results with antioxidant assays showed that the methanolic extract, the n-butanolic fraction and the isolated flavonoids from M. albicans had a significant scavenging capacity against AAPH and DPPH. Quercetin, quercetin-3-O-glucoside, rutin, 3-(E)-p-coumaroyl-α-amyrin was isolated from the n-butanolic fraction and α-amyrin, epi-betulinic acid, ursolic acid, epi-ursolic acid from the chloroformic extract. The results presented in this study demonstrate that M. albicans is a promising species in the search for biologically active compounds.
Highlights
IntroductionThere has been growing interest in plants as new therapeutic agents
Over the past decades, there has been growing interest in plants as new therapeutic agents
Total phenolic (TP) contents was expressed in gallic acid equivalents (GAE) equivalents using the equation based on the gallic acid calibration curve: y = 0.0022x + 0.051 (R2 = 0.9978), where y is absorbance at 760 nm and x is gallic acid concentration in mg/L
Summary
There has been growing interest in plants as new therapeutic agents. Free radicals found in oxygen or, less reactive oxygen species (ROS), are products of the normal metabolism of cells They are associated with the production of energy, phagocytosis, regulation of cellular growth, inter-cellular signaling, and the synthesis of important biological substances. Free radicals are associated with a series of pathologies, such as arthritis, hemorrhagic shock, cardiac disease, cataracts, cognitive disfunctions, aging, and cancer, and may be considered the cause or the aggravating factor to the disease conditions [4] These observations have encouraged the search for new chemical substances of plant origin, especially flavonoids, with antioxidant potential. The methanolic extracts of Miconia albicans, Miconia cabucu, Miconia rubiginosa, Miconia stenostachya and the chloroformic extract of M. albicans were evaluate, in vivo, to genotoxic and mutagenic effects using the comet assay and micronucleus test. It describes the isolation, purification, and structural elucidation of the compounds isolated by the methanolic and chloroformic extracts
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