Antioxidant activity and protection against oxidative-induced damage of Acacia shaffneri and Acacia farnesiana pods extracts: in vitro and in vivo assays.

Claudia Delgadillo Puga,Mario Cuchillo Hilario,José Pedraza Chaverri,Marsela Alejandra Álvarez Izazaga,Eduardo Molina Jijón,Omar Medina Campos,José Ángel Ledesma Solano,Margarita Díaz Martínez,José Guillermo Espinosa Mendoza

doi:10.1186/s12906-015-0959-y

Abstract

BackgroundObesity is a worldwide public health issue, reaching epidemic condition in developing countries associated to chronic diseases. Oxidative damage is another side effect of obesity. Antioxidant activity from plant components regulates at some extent this imbalance. Main goal of the present study was to determine the antioxidant activity and protection against oxidative-induced damage of Acacia shaffneri (AS) and Acacia farnesiana (AF) pods extracts.MethodsTo evaluated antioxidant activity and radical scavenging capacity of AS and AF extracts, two experiments were performed: 1) pods extracts were challenged against H2O2 using kidney cells in an in vitro assay; and 2) (Meriones unguiculatus) was employed in an in vivo assay to observe the effect of pods extracts on scavenging properties in plasma.ResultsBoth pods extracts presented an important protective effect on radical scavenging capacity against ABTS• + and DPPH+, and also in TBARS formation in vitro. Vegetal pods extracts did not induce any pro-oxidative effect when added to kidney cells in DMEM. Cells damage in DMEM with addition of H2O2 was significantly higher than those when vegetal pods extracts were added at 50 (P < 0.05) or 200 ppm (P < 0.001). Plasma scavenging properties presented an important dose-dependent positive effect in those groups where pods extracts were administered.ConclusionsThe antioxidant protection of the acacia pods extracts reported in this study suggests the possible transference of antioxidant components and protective effects to animal products (milk, meat, and by-products) from Acacia pods when this vegetation is included in the diet. In order to evaluate, the possible transference of theirs antioxidant components to animal products, the incorporation of these non-conventional resources to ruminant feeding is a good opportunity of study. Profiling of Acacia farnesiana pods extract is necessary to identify the responsible bioactive compounds of protective properties.

Highlights

Obesity is a worldwide public health issue, reaching epidemic condition in developing countries associated to chronic diseases
Antioxidant activity from plant components regulates at some extent this imbalance by increasing the scavenging activity of reactive oxygen species (ROS) such as hydrogen peroxide (H2O2)
On ABTS + radical scavenging capacity no significant difference was found between extracts (P > 0.05)

Summary

Introduction

Obesity is a worldwide public health issue, reaching epidemic condition in developing countries associated to chronic diseases Oxidative damage is another side effect of obesity. Many plants distributed in arid and semi-arid environments have alternative uses such as forage resources; traditional medicine and more recently, they have been pointed out as antioxidant sources [3,4,5], mainly due to their polyphenol content These compounds have received attention because of their antioxidant capabilities and their protection against ROS-induced damage [2, 6] with biological functions like antiinflammatory, anticancer, antiobesity, antiaging [7], hepatoprotective and antidiabetic properties [8]. The extracts obtained from these plants have previously shown antimicrobial [3], antihelmintic [9] and antioxidant [4] properties

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