Abstract

The objectives of this study were to evaluate antioxidant activity, qualitative phytochemical analysis and LC-MS/MS analyses of aqua-ethanol extract of Bauhinia racemosa Lam. plant leaves. The extract afforded the rich content of different phytochemicals reported in qualitative phytochemical analysis, LC-MS/MS spectral analysis the leaves and antioxidant activity pharmacological activity. The extract was subjected to assess their antioxidant potential using DPPH in vitro system. Using DPPH (2, 2-Diphenyl-1-picrylhydrazyl) free radical scavenging assay, the aqua-ethanol extract was found to have significant antioxidant activity with an IC50 value 42.17 ± 0.03 mg/mL. A qualitative phytochemical analysis shows that the plant leaves contains carbohydrate, protein, amino acids, glycoside, tannins, saponin, flavonoids and phenolic compounds. It is concluded that the aqua-ethanol extract of Bauhinia racemosa Lam. leaves have been strong antioxidant potential. Further study is needs for isolate and characterizes the active antioxidants, which may serve as a potential source of natural bioactive antioxidants.

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