Antioxidant Activity and Mechanism of Resveratrol and Polydatin Isolated from Mulberry (Morus alba L.).

Ziwei Li,Xiaoman Chen,Jun Li,Jinglin Zhang,Guo Liu,Yong Cao,Jianyin Miao

doi:10.3390/molecules26247574

Abstract

Natural stilbenes have unique physiological effects, such as anti-senile dementia, anti-cancer, anti-bacterial, lowering blood lipid, and other important biological functions, which have attracted great attention from scholars in recent years. In this study, two stilbene compounds, resveratrol (RES) and polydatin (PD), were isolated from Mulberry (Morus alba L.), and their antioxidant activity and mechanism were investigated. The results showed that the contents of RES and PD in mulberry roots were 32.45 and 3.15 μg/g, respectively, significantly higher than those in mulberry fruits (0.48 and 0.0020 μg/g) and mulberry branches (5.70 and 0.33 μg/g). Both RES and PD showed high antioxidant potential by DPPH, ABTS free-scavenging methods, and ORAC assay, and provided protection against oxidative damage in HepG2 cells by increased catalase (CAT) activity, superoxide dismutase (SOD) activity, and Glutathione (GSH) content, and decreasing generation of reactive oxygen species (ROS), lactate dehydrogenase (LDH) level, and malondialdehyde (MDA) content. Therefore, RES and PD treatment could be effective for attenuating AAPH-induced oxidative stress in HepG2 cells. This study will promote the development and application of stilbene compounds. Furthermore, the RES and PD could be used as antioxidant supplements in functional foods, cosmetics, or pharmaceuticals, contributing to health improvement.

Highlights

There is an increasing demand for the beneficial effects of natural-source functional products and their biologically active molecules [1]
We found that the content of PD in mulberry roots (3.15 μg/g) was the highest, which was 9.6 times and 1576.7 times that in mulberry branches (0.33 μg/g and mulberry fruits (0.0020 μg/g) (Table 1)
Based on the above results, we found that RES and PD showed better protective effects when the cells were treated to a lower degree of oxidative damage

Summary

Introduction

There is an increasing demand for the beneficial effects of natural-source functional products and their biologically active molecules [1] Functional ingredients such as polyphenols, bioactive peptides, probiotics, vitamins, and minerals are directly added to various foods to enhance their potential to prevent or control various diseases [2,3]. Among these bioactive compounds, plant-based functional foods have received significant attention in the food industry in recent times. Polydatin (3,40 ,5-trihydroxystibene-3-β-mono-D-glucoside, PD), as the most abundant derivative of RES in nature, has attracted attention in the pharmaceutical and functional food industries ascribed to its higher oral bioavailability [12]

Methods

Results

Conclusion