Abstract
The present study compares the protective properties of aqueous extracts of six medicinal plants, Phyllanthus emblica, Terminalia chebula (black and yellow), Terminalia arjuna, Balsamodendron Mukul and Alium sativum against lipid per-oxidation in mice brain. The antioxidant activities were analyzed by lipid per-oxidation assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical assay, total antioxidant activity and metal chelation. The extracts (fruits and bark) showed inhibition against thiobarbituric acid reactive species (TBARS) induced by pro-oxidant (10 µM FeSO4) in mice brain. Moreover, the free radical scavenging activities of the extracts was evaluated by the scavenging of DPPH radical (IC₅₀, 23.23 ± 1.2 µg/ml (Phyllanthus emblica), 20.24 ± 0.9 µg/ml (Terminalia chebula yellow) and 17.33 ± 1.1 µg/ml (Terminalia chebula black), 19.44 ± 0.45 µg/ml (Terminalia arjuna), 56.59 ± 2.1 µg/ml (Balsamodendron Mukul) and < 200 µg/ml (Alium sativum). The higher antioxidant and inhibitory effect of Terminalia chebula black in this study could be attributed to its significantly higher phenolic content, Fe(II) chelating ability, reducing ability and free radical scavenging activity. Therefore oxidative stress in brain could be potentially prevented by the intake of these plants.
Highlights
Reactive oxygen species (ROS) are generated spontaneously in cells during metabolism and are implicated in the aeitology of different degenerative diseases, such as heart diseases, stroke, rheumatoid arthritis, diabetes and cancer (Halliwell et al, 1992)
The results revealed that treatment with Fe(II) caused a significant (P < 0.05) increase in thiobarbituric acid reactive substances (TBARS) compared to the basal
Three separate controls were used for Phyllanthus emblica, Terminalia chebula yellow and Terminalia chebula black (Figure 1)
Summary
Reactive oxygen species (ROS) are generated spontaneously in cells during metabolism and are implicated in the aeitology of different degenerative diseases, such as heart diseases, stroke, rheumatoid arthritis, diabetes and cancer (Halliwell et al, 1992). Oxidative stress results from either a decrease of natural cell antioxidant capacity or an increased amount of reactive oxygen species (ROS) in organisms. It is well established, that free radicals are associated with processes that lead to cell degeneration, especially in the brain (Shulman et al, 2004). The present study compares the protective properties of aqueous extracts of six medicinal plants, Phyllanthus emblica, Terminalia chebula (black and yellow), Terminalia arjuna, Balsamodendron Mukul and Alium sativum against lipid per-oxidation in mice brain. The free radical scavenging activities of the extracts was evaluated by the scavenging of DPPH radical (IC50, 23.23±1.2 μg/ml (Phyllanthus emblica), 20.24±0.9 μg/ml (Terminalia chebula yellow) and 17.33±1.1 μg/ml (Terminalia chebula black), 19.44±0.45 μg/ml (Terminalia arjuna), 56.59±2.1 μg/ml (Balsamodendron Mukul) and 200 μg/ml (Alium sativum). Oxidative stress in brain could be potentially prevented by the intake of these plants
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