Abstract
Hypericum japonicum is traditionally used as a folk medicine to treat cholestasis and hepatitis. Quercetin 7-rhamnoside (Q7R) is one of the main flavonoid components of Hypericum japonicum and has been rarely studied. The aim of the present study was to evaluate the antioxidant activity and hepatoprotective potential of Q7R. In the in vitro experiments, DPPH, ABTS and ferric reducing antioxidant power (FRAP) assays were first performed to assess the antioxidant properties of Q7R, and then a H2O2-induced oxidative damage cellular model was used to determine the cytoprotective and antioxidant properties of Q7R in human liver L-02 cells. In the in vivo experiment, the hepatoprotective activity of Q7R was evaluated by carbon tetrachloride (CCl4)-induced liver damage model in mice. The results of the three in vitro assays (DPPH, ABTS and FRAP) demonstrated that Q7R significantly exhibited antioxidant activity. The cell experiment results showed that Q7R possessed cytoprotective and antioxidant effects on H2O2-treated L-02 cells. In the in vivo experiments, Q7R suppressed the up-regulation of serum activities of ALT, AST, LDH and triglyceride (TG) levels with dose-dependency. Q7R down-regulated the production of MDA and increased the hepatic GSH content and antioxidant enzymes CAT activities. Hepatic morphological analysis was also performed to confirm the biochemical changes. In summary, these results suggested that Q7R could be considered as a potential source of natural antioxidants, and may become a promising candidate for the treatment of liver injury in the future.
Highlights
Lots of liver damage, ranging from subclinical icteric hepatitis to necroinflammatory hepatitis, cirrhosis and carcinoma, have been proven to associate with redox imbalance and oxidative stress [1,2].The liver metabolizes various compounds that produce reactive oxygen radicals (ROS)
The antioxidant activity of flavonoids are due to their capacity to transfer substances, have long been reported as antioxidants in plants, which is due to their ability to scavenge electrons free radicals, chelate metal catalysts, activate antioxidant enzymes, reduce alpha-tocopherol free radicals and to reduce free radical formation [6,7]
Several reports have indicated that an important mechanism of hepatoprotective effects may be related to their capacity to transfer hydrogen to free radicals, activate antioxidant enzymes and inhibit oxidases [22,23,24,25]
Summary
Lots of liver damage, ranging from subclinical icteric hepatitis to necroinflammatory hepatitis, cirrhosis and carcinoma, have been proven to associate with redox imbalance and oxidative stress [1,2]. The antioxidant activity of flavonoids are due to their capacity to transfer substances, have long been reported as antioxidants in plants, which is due to their ability to scavenge electrons free radicals, chelate metal catalysts, activate antioxidant enzymes, reduce alpha-tocopherol free radicals and to reduce free radical formation [6,7] Since these compounds are based on the radicals, and inhibit oxidases [12]. Flavan nucleus, the number, positions, and types of substitutions influence radical scavenging and They havejaponicum, multiple biological activities, including antioxidant activity [9],used for the treatment of cholestasis, well as acute and chronic hepatitis [13].immune-stimulating. Reported that Schouwia electrons free radicals, acute chelatehepatotoxicity metal catalysts, activate antioxidant enzymes, reduce alpha-tocopherol radicals, and inhibit oxidases [12].Q7R significantly exerted curative effects on CCl4-induced liver thebaica.
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