Antioxidant Activity and Acteoside Analysis of Abeliophyllum distichum.

Hak-Dong Lee,Pil-Mun Jung,Sanghyun Lee,Qi Qi Pang,Ji Hyun Kim,Eun Ju Cho

doi:10.3390/antiox9111148

Abstract

This study determined acteoside and its content in Abeliophyllum distichum via HPLC/UV and LC/ESI-MS to obtain insights into the potential use of this plant as an antioxidant agent. Moreover, 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (•OH), and O2− radical scavenging activity assays were performed to assess in vitro antioxidative activity. The DPPH, •OH, and O2− radical scavenging activities of A. distichum leaf EtOH extracts at a 250 μg/mL concentration were 88.32%, 94.48%, and 14.36%, respectively, whereas those of stem extracts at the same concentration were 88.15%, 88.99%, and 15.36%, respectively. The contents of acteoside in A. distichum leaves and stems were 162.11 and 29.68 mg/g, respectively. Acteoside was identified as the main antioxidant compound in A. distichum leaves, which resulted in DPPH, •OH, and O2− radical scavenging activities of 82.84%, 89.46%, and 30.31%, respectively, at a 25 μg/mL concentration. These results indicate that A. distichum leaves and stems containing the antioxidant acteoside can be used as natural ingredients for functional and nutritional supplements.

Highlights

Abeliophyllum distichum Nakai is an important plant resource and represents the only species within the genus Abeliophyllum in the world [1]
Many researchers have focused on the development and identification of natural antioxidant products
We identified a flavonoid from A. distichum and evaluated the in vitro antioxidant activities of its extracts as well as its active compound, acteoside

Summary

Introduction

Abeliophyllum distichum Nakai is an important plant resource and represents the only species within the genus Abeliophyllum in the world [1]. A. distichum is an endemic plant in Korea and is commonly referred to as white forsythia This plant is protected and has been designated as an endangered plant species in Buan-, Goesan-, and Yeongdong-gun, Korea [2,3], with Goesan-gun being the main producer of this resource. It has been used as a landscape plant, A. distichum has been found to possess therapeutic value due to its anti-cancer [4], anti-diabetic (via aldose reductase inhibition) [5], and anti-hypertensive properties [3]. The crude ash contents of A. distichum leaves and stems are 1.32% and 0.91%, respectively, and its fructose and glucose contents have been reported as 32.13 and 56.17 mg/g for the leaves, and 11.38 and 10.59 mg/g for the stems, respectively [14]

Methods

Results

Conclusion