Abstract
Several medicinal foods abound in traditional medicine with antioxidant potentials that could be of importance for the management of several diseases but with little or no scientific justification to substantiate their use. Thus, the objective of this study was the assessment of the antioxidant effect of two leave extracts of Solanum nigrum L. (SN), which is a medicinal plant member of the Solanaceae family, mainly used for soup preparation in different parts of the world. Then methanolic/water (80:20) (SN1) and water (SN2) leaves extracts were prepared. The total polyphenolic content and the concentration of phenolic acids and flavones compounds were determined. In order to verify whether examined extracts were able to restore the oxidative status, modified by glutamate in primary cultures of astrocytes, the study evaluated the glutathione levels, the intracellular oxidative stress, and the cytotoxicity of SN1 and SN2 extracts. Both extracts were able to quench the radical in an in vitro free cellular system and restore the oxidative status in in vitro primary cultures of rat astroglial cells exposed to glutamate. These extracts prevented the increase in glutamate uptake and inhibited glutamate excitotoxicity, which leads to cell damage and shows a notable antioxidant property.
Highlights
Several medicinal foods abound in traditional medicine with antioxidant activities
The total phenolic contents was assayed by a modified Folin-Ciocalteu method using gallic acid, and is reported in Table 1, with the physical characteristics and the dry weight yields of SN1 and SN2
In our previous research studies, using an experimental model of excitotoxicity, we demonstrated that glutamate exposure in primary cultures of astrocytes might be part of the biochemical response to oxidative stress induced by a prolonged exposure of astrocyte cultures to the neurotransmitter [46]
Summary
Several medicinal foods abound in traditional medicine with antioxidant activities. This could be of important for the management of several diseases but has little or no scientific justification to substantiate their use. As in Asia and in sub-Saharan Africa, green leafy vegetables are used as one of the major components of local dishes. (SN) belongs to the Solanaceae family to Europe, Asia, and North America and was introduced in South America, Australia, and Africa. It represents one of the largest and most variable species groups of the genus. SN, well known as “Black Nightshade” (the English name), is an herbal plant widely distributed throughout the world, extending from tropical regions to temperate regions [1]
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