Antioxidant Activities of Polyphenols Extracted from Perilla frutescens Varieties

Linghua Meng,Yves F Lozano,Emile M Gaydou,Bin Li

doi:10.3390/molecules14010133

Abstract

Various cultivars of Perilla frutescens (L.) (var. crispa and var. frutescens) Britt. were harvested in China and Japan. They were easily differentiated on the basis of their foliage color, that varied from red to green. Water extracts of dried plants were investigated for their antioxidant activity (AA) and their polyphenolic compounds compared. Among them, cinnamic acid derivatives (coumaroyl tartaric acid, caffeic acid and rosmarinic acid), flavonoids (apigenin 7-O-caffeoylglucoside, scutellarein 7-O-diglucuronide, luteolin 7-O-diglucuronide, apigenin 7-O-diglucuronide, luteolin 7-O-glucuronide, and scutellarein 7-O-glucuronide) and anthocyanins (mainly cis-shisonin, shisonin, malonylshisonin and cyanidin 3-O-(E)-caffeoylglucoside-5-O-malonylglucoside) were quantified. AA assays are based on the inhibition of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The DPPH radical scavenging activity was calculated as Trolox® [(±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid] equivalent antioxidant capacity (TEAC). The mean amount of total phenolics of the water extracts (4-29 µmol/100 mL) and the TEAC value calculated (23-167 µmol TE/100 mL) confirmed the high antioxidant activity of these leaf water extracts. These results were highly correlated within some o-dihydroxylated polyphenolic compounds and AA.

Highlights

Perilla frutescens (L.) Britt. (Lamiaceae) is an edible plant frequently used in Asian countries such as China, Korea and Japan [1]
The present study aimed to evaluate the total polyphenolic contents, and the antioxidant capacities of eight water extract samples of P. frutescens varieties belonging to the var. frutescens, and var. crispa, and collected from various growing areas including China and Japan
Water-soluble polyphenolics were extracted from the dried leaves and analyzed by DAD-HPLC

Summary

Introduction

Perilla frutescens (L.) Britt. (Lamiaceae) is an edible plant frequently used in Asian countries such as China, Korea and Japan [1]. The present study aimed to evaluate the total polyphenolic contents (cinnamic, flavonic and anthocyanic derivatives), and the antioxidant capacities of eight water extract samples of P. frutescens varieties belonging to the var. Eight leaf samples of P. frutescens (Table 1) were collected from various area of China (seven samples) and one sample from Japan. Water-soluble polyphenolics were extracted from the dried leaves and analyzed by DAD-HPLC.

Results

Conclusion