Antioxidant Activities of Green Synthesized Zinc Oxide Nanoparticles and its Curative Influence in Carbon tetrachloride‐Induced Hepatorenal Damage in Adult Rats

Abstract

The objective of this study was to synthesize Zinc Oxide nanoparticles coated with Pterocarpus mildbraedii leaf extracts (PmZnONPs) by an eco‐friendly method, and investigate its antioxidant activity and curative potential against carbon tetrachloride (CCl4) ‐ induced hepatorenal toxicity in rats. PmZnONPs was characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), fourier transform infra‐red spectroscopy (FTIR), alongside zeta potential measurement. In vitro, free radical activities using 1, 1‐diphenyl‐2‐ picrylhydrazyl (DPPH) and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) showed strong antioxidant activity compared to ascorbic acid. In rats, PmZnONPs (1 and 3 mg/kg p.o.) significantly prevented CCl4‐induced elevations of alkaline phosphatase, gamma‐glutamyl transferase, creatinine, urea, aspartate aminotransferase, alanine aminotransferase, and total bilirubin. Moreover, PmZnONPs restored the decreased levels of plasma uric acid, hepatorenal antioxidant enzymes, including superoxide dismutase, glutathione peroxidase, glutathione transferase, and glutathione, that were significantly induced by CCl4 treatment. Histological studies indicated that CCl4‐induced lesions in the liver and kidney were alleviated by the treatment of PmZnONPs. Immunohistochemical studies showed that PmZnONPs significantly suppressed the high immunoreactivity of cyclooxygenase‐2, interleukin‐6 and NF‐κB arising from CCl4 intoxication. These results indicated that PmZnONPs protected the rats from CCl4‐induced hepatorenal damage via its antioxidant properties as well as its inhibition of pro‐inflammatory proteins.Support or Funding InformationThis research did not receive any support from any funding organization.