Antioxidant activities of Geranium sanguineum L. polyphenolic extract in chemiluminescent model systems

Abstract

The aim of this study is to assess the antioxidant activities of Geranium sanguineum polyphenolic extract in chemiluminescent model systems by calculating the constants of inhibition towards different reactive oxygen species (ROS). The kinetics of lucigenin-enhanced chemiluminescence was examined in three chemical systems designed for generation of ROS (OH, O2−, H2O2) and in one system with FeSO4 added as a chelator. Antioxidant enzyme activities of superoxide dismutase and glutathione reductase were also evaluated. All the calculated constants of inhibition were compared to vitamin C and vitamin E values obtained earlier. Based on the results we can make the following major conclusions:1)the polyphenol complex reacts against all studied ROS (H2O2, OH, O2−) with various efficacy. It exerts lower anti-oxidant effects when compared to standards such as vitamin C and vitamin E.2)the polyphenol complex has pronounced chelation properties against Fe2+, which is of extreme biological importance.3)The reference concentration (0.1 mg/ml) is most appropriate for therapeutic purposes.All obtained data contribute to the elucidation on the therapeutic and prophylactic reactive mechanisms of polyphenols extracted from Geranium sanguineum L.