Abstract
This study was carried out to investigate the total phenolic compounds beside the potential antioxidant activity of Guiera senegalensis and Combretum hartmannianum extracts. The content of total phenolics in the extracts was determined spectrometrically according to the Folin-Ciocalteu procedure and calculated as gallic acid equivalents (GAE). The antioxidative activities by beta-carotene linoleic acid and DPPH methods of extracts from Guiera senegalensis leaves, (GSL), Guiera senegalensis roots (GSR) and Combretum hartmannianum leaves (CHL), were evaluated in sunflower oil in the dark at 70°C. Total phenolic compounds were 240.1, 275.6 and 253.4 mg/g respectively. The methanolic extracts of (GSL), (GSR) and (CHL), were markedly effective in inhibiting the oxidation of linoleic acid and subsequent bleaching of s-carotene in comparison with the control. Plants extracts (500 mg) effectively inhibited the formation of peroxides in sunflower oil and showed the highest antioxidative activity compared with 20 mg BHA. The GSL extract is the most effective followed by the GSR and CHL extract. The antioxidant activity of the extracts measured by DPPH free radical showed high reduction of 50% DPPH in CHL extract followed by GSR and GSL. Clear differences were found between the control and sunflower oil containing C. hartmannianum and G. senegalensis extracts or BHA, which decreased and slowed the rate of peroxide formation resulting in lower PVs after 96 h of storage at 70 °C.
Highlights
Plants have been studied as sources of different classes of natural antioxidants.Some plants, grown wild or cultivated, have been exploited commercially for many years (Exarchou et al, 2002).Guiera senegalensis family combretaceae known locally in Sudan as Ghibaish is a shrub, up to 3 m high, leaves were used for leprosy prevention, the root decoction is used to cure diarrhea, dysentery.Combretum hartmannianum a shrub up to 4 m, as a tree under favorable conditions 10 m high known locally inSudan as Habeel
The results showed that Guiera senegalensis roots (GSR) has the highest amount of total phenolic compounds followed by Combretum hartmannianum leaves (CHL) and GSL
The PVs of sunflower oil containing 1000 mg GSL, GSR, CHL or 20 mg butylated hydroxyanisole (BHA) were found as 47.0, 21.5, 23.5, and 23.0 meq O2/kg, respectively (Fig.4). These samples showed an inhibition of oxidation (IO) after 96 hours of storage of 18.3, 67.0, 63.3, and 67.3% respectively, in comparison to a control GSR showed the highest activity, but all the extracts showed less inhibition of oxidation than BHA
Summary
Plants have been studied as sources of different classes of natural antioxidants.Some plants, grown wild or cultivated, have been exploited commercially for many years (Exarchou et al, 2002).Guiera senegalensis family combretaceae known locally in Sudan as Ghibaish is a shrub, up to 3 m high, leaves were used for leprosy prevention, the root decoction is used to cure diarrhea, dysentery.Combretum hartmannianum a shrub up to 4 m, as a tree under favorable conditions 10 m high known locally inSudan as Habeel. Plants have been studied as sources of different classes of natural antioxidants. Grown wild or cultivated, have been exploited commercially for many years (Exarchou et al, 2002). Guiera senegalensis family combretaceae known locally in Sudan as Ghibaish is a shrub, up to 3 m high, leaves were used for leprosy prevention, the root decoction is used to cure diarrhea, dysentery. Combretum hartmannianum a shrub up to 4 m, as a tree under favorable conditions 10 m high known locally in. Leaves alternate, shining light green when young, typically rust-colored when mature, leaves were used as an antipyretic, diuretic and for various diseases such as yellow fever, hepatic disorder (Maydell 1990). The methanolic extracts of different parts of Combretum hartmannianum possessed significant activity against the chloroquine-sensitive
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