Antioxidant activities of celery and parsley juices in rats treated with doxorubicin.

Jovanka Kolarovic,Janka Zlinská,Svetlana Trivic,Matilda Vojnovic,Mira Popovic

doi:10.3390/molecules15096193

Abstract

We have examined the influence of diluted pure celery and parsley leaf and root juices and their combinations with doxorubicin on the antioxidant status [as measured by the content of reduced glutathione (GSH) and ferric reducing antioxidant power (FRAP)] in liver homogenate and hemolysate and on the contents of cytochrome P450 in liver homogenate. It was found that doxorubicin significantly decreased the content of reduced glutathione and the total antioxidative status (FRAP) in liver homogenate and hemolysate, while celery and parsley juices alone and in combination with doxorubicin had different actions. Doxorubicin and celery juice had no effect on content of cytochrome P450. However, in combination with doxorubicin, parsley root juice significant increased, and parsley leaves juice decreased the cytochrome P450 content (compared to doxorubicin treated animals). Only parsley root juice significantly increased the content of cytochrome P450.

Highlights

Doxorubicin is among the most effective and widely used cytotoxic drugs from the anthracycline group
We investigated the effect of treatment with doxorubicin on the cytochrome P450 content
In blood hemolysate reduced glutathione content was significantly decreased by parsley leaf juice, doxorubicin and by a combination of doxorubicin and parsley leaf juice (Figure 2)

Summary

Introduction

Doxorubicin is among the most effective and widely used cytotoxic drugs from the anthracycline group. Linnaeus classified it Apium petroselinum L.) contains flavonoids (apiin and luteolin) and essential oil (apiol and myristicin), responsible for both the medical uses and toxicity of parsley. Our previous results have pointed at antioxidant and hepatoprotective effects of celery and parsley extracts, as well as influence on pharmacodynamic activities [7]. These findings have led us to examine the influence of celery and parsley root and leaf juices on total antioxidant status and content of reduced glutathione in liver homogenate and hemolysate in doxorubicin treated rats. We investigated the effect of treatment with doxorubicin (alone and in combination with celery and parsley roots and leaves juices) on the cytochrome P450 content

Results and Discussion

Plant Material

Animal Treatment

Biochemical Assays

Chemicals

Conclusions