Antioxidant activities of artesunate-procyanidin hybrid compound in erythrocyte and liver of Plasmodium berghei NK65-infected mice.

Abstract

Resistance of malaria parasites to conventionally used antimalarial drugs has necessitated the search for new potent antimalarials, especially those that can also ameliorate oxidative stress-mediated secondary complications. This has led to the synthesis of an antimalarial artesunate-procyanidin hybrid compound (PC14), but it has not been evaluated for its antioxidant activity. This study was carried out to evaluate the antioxidant activities of PC14 in the erythrocyte and liver of Plasmodium berghei NK65-infected mice. A hundred mice were randomly divided into 10 equal groups (A-J). Mice in Groups B-J were inoculated with P.berghei NK65 while group A mice were not inoculated. Starting from Day 3 post-inoculation, dimethyl sulfoxide (DMSO) (5%) was administered to mice in Groups A and B (normal and negative controls, respectively), while various doses of chloroquine, artesunate, procyanidin, and PC14 were administered to their respective groups for 3 days. Thereafter, antioxidant parameters were determined in the erythrocyte and liver on Days 6 and 10 post-inoculation. A significant increase (P < 0.05) was observed in malondialdehyde levels in the erythrocyte and liver of negative control on Day 10 post-inoculation compared to normal controls. Significant reduction (P < 0.05) was observed in activities of liver catalase and superoxide dismutase and erythrocyte glutathione peroxidase and glutathione-S-transferase of negative control on Days 6 and 10 compared to normal controls. However, PC14 at various doses significantly (P < 0.05) reversed these alterations. The results suggest that PC14 possesses antioxidant activity, and it enhanced antioxidant defense in the erythrocyte and liver of P.berghei-infected mice.