Abstract
Obesity is one of the most common metabolic diseases resulting in metabolic syndrome. In this study, we investigated the antiobesity effect of Gentiana lutea L. (GL) extract on 3T3-L1 preadipocytes and a high-fat-diet (HFD)-induced mouse model. For the induction of preadipocytes into adipocytes, 3T3-L1 cells were induced by treatment with 0.5 mM 3-isobutyl-1-methylxanthine, 1 mM dexamethasone, and 1 μg/mL insulin. Adipogenesis was assessed based on the messenger ribonucleic acid expression of adipogenic-inducing genes (adiponectin (Adipoq), CCAAT/enhancer-binding protein alpha (Cebpa), and glucose transporter type 4 (Slc2a4)) and lipid accumulation in the differentiated adipocytes was visualized by Oil Red O staining. In vivo, obese mice were induced with HFD and coadministered with 100 or 200 mg/kg/day of GL extract for 12 weeks. GL extract treatment inhibited adipocyte differentiation by downregulating the expression of adipogenic-related genes in 3T3-L1 cells. In the obese mouse model, GL extract prevented HFD-induced weight gain, fatty hepatocyte deposition, and adipocyte size by decreasing the secretion of leptin and insulin. In conclusion, GL extract shows antiobesity effects in vitro and in vivo, suggesting that this extract can be beneficial in the prevention of obesity.
Highlights
Obesity is the most common metabolic disease associated with several metabolic complications, including type 2 diabetes, hypertension, and cardiovascular disease [1]
Adipogenesis contributes to excess fat accumulation in adipocytes during the differentiation of preadipocytes
Adipogenesis was assessed by the messenger ribonucleic acid expression of adipogenic-inducing genes, including adiponectin (Adipoq), CCAAT/enhancer-binding protein alpha (Cebpa), and glucose transporter type 4 (Slc2a4)
Summary
Obesity is the most common metabolic disease associated with several metabolic complications, including type 2 diabetes, hypertension, and cardiovascular disease [1]. Obesity is a disorder related to the imbalance of energy intake and expenditure, resulting in excess lipid accumulation in white adipose tissue [2]. Long-term moderated lifestyle changes such as decreasing food intake and increasing physical activity have successfully reduced body weight in humans [3]. Nutrition and exercise intervention are considered as effective strategies to prevent and manage obesity [4]. Pharmacological drugs for the treatment of obesity can reduce appetite or decrease fat absorption [5]. The long-term use of pharmacotherapies for weight loss still has limitations with side effects
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
