Antiobesity activity of ethanolic extract ofCitrus maximaleaves on cafeteria diet induced and drug induced obese rats

Abstract

Two doses 200 mg/kg and 400 mg/kg of Citrus maxima leaves extract (oral route) were subjected for the evaluation of anti-obesity activity against cafeteria diet and olanzapine induced obesity in rats. Wistar Albino rats (150 to 200 g) and albino mice (20-25 g) of either sex were used for this study. These animals were maintained under standard conditions (temperature 22 ± 2°C, relative humidity 60±5% and 12 h light/dark cycle) and had free access to standard pellet diet and water ad libitum. The leaves were washed with double distilled water, shade dried and powered and was extracted with 80% ethanol for 72h in a cold percolation method. After that the solvent was evaporated under pressure to get the crude extract i.e. Ethanolic Extract of Citrus Maxima (EECM). Orlistat (10mg/kg oral) was served as standard in both models. Body weight, body temperature, were measured and evaluated. Serum parameters such as TG, TC, HDL, LDL, VLDL, SGOT, SGPT were also evaluated. Liver was isolated to evaluate liver weight and triglyceride content. Both the lower dose (200mg/kg) and higher dose (400mg/kg) of Citrus maxima leaves showed significant decrease in body weight, body temperature, serum parameters and liver weight when compared to obese control group. All treated animals showed significant decrease in serum level. The results obtained were comparable with that of standard. The present study concluded that Citrus maxima leaves were found to be effective against Cafeteria diet and olanzapine induced obesity.