Anti‐nonspecific adsorption segments‐assisted self‐driven surface imprinted fibers for efficient protein separation

Abstract

AbstractAt present, the development of high‐performance protein imprinted materials is still a research hotspot in the field of protein imprinting. Herein, anti‐protein adsorption segment (CBMA)‐assisted self‐driven bovine serum albumin (BSA) surface imprinted fibers MTCFs@SIP@CBMA with high recognition selectivity are pioneered using the strategies of combining magnetic nanomaterial surface imprinting technique with amino‐Michael addition. The special structure of the carrier MTCFs endows MTCFs@SIP@CBMA with magnetic performance and self‐driven adsorption performance, which simplifies the separation process while improving the adsorption capacity and accelerating the adsorption rate. The adsorption capacity for BSA reached 390 ± 20 mg/g within 30 min. The introduction of CBMA segments on the surface after imprinting by amino‐Michael addition makes its polymer chain length and position controllable. Under the strongest anti‐nonspecific adsorption effect, MTCFs@SIP@CBMA exhibit excellent specific identification to BSA from mixed proteins. Additionally, MTCFs@SIP@CBMA show considerable reusability. Therefore, MTCFs@SIP@CBMA are expected to be applied in efficient separation of proteins in biological samples.