Abstract
In the present study, the antinociceptive effects of acute (2, 4 and 6 ml/kg) and chronic (1, 2 and 3 ml/kg for 14 days)oral administration of pomegranate (Punica granatum L.) juice and seed extract with or without morphine and naloxane were investigated on hypertonic saline-induced acute corneal pain perception in mice. The number of eye wipes with a forelimb was counted for a period of 30 seconds as the criterion for pain assessment. Acute oral administration of the extract (at 6 ml/kg dose, once) and chronic oral administration (at 2 and 3 ml/kg for 14 days each) significantly decreased the number of eye wipes after subcutaneous injection of morphine (2 mg/kg, sc), naloxone (2 mg/kg, sc) and normal saline (2 mg/kg, sc) compared with control (p < 0.05). The morphine-induced antinociception was significantly improved by both acute and chronic oral administrations of pomegranate extract (p < 0.05). Naloxone (2 mg/kg, sc) did not reverse the antinociceptive effects of acute (at 6 ml/kg dose, once, oral) and chronic (at 2 and 3 ml/kg for 14 days each) treatments. These findings demonstrate that acute high-dose and long-term lower-dose of pomegranate juice and seed extract can decrease acute corneal pain and improve morphine-induced antinociception in mice.
Highlights
Pomegranate, Punica granatum L., has a wide range of therapeutic effects that may be attributed to anti-oxidant, anti-carcinogenic, and anti-inflammatory mechanisms
Mice were treated by the current regulations of Medical Science Research Center, Tehran Medical Branch, Islamic Azad University, Tehran, Iran, in accordance with the National Institutes of Health (NIH) Guidelines for Care and Use of Laboratory Animals
None of them reacted to topical application of 0.15 M NaCl solution (Were not shown in the figure)
Summary
Pomegranate, Punica granatum L., has a wide range of therapeutic effects that may be attributed to anti-oxidant, anti-carcinogenic, and anti-inflammatory mechanisms. Antinociceptive effects of many Persian medicinal plants, olive (Olea europaea L.) [5], ginger (Zingiber officinale Roscoe) [6], saffron (Crocus sativus L.) [7], oleaster (Elaeagnus angustifolia) [8], fennel (Foeniculum vulgare) [9] and black cumin seed (Nigella sativa L.) were investigated in experimental studies and even in clinical trials [10]. The eye wiping with a forelimb during acute hypertonic saline-induced pain (one 40 μl drop of 5 M NaCl solution topically on the corneal surface) has been introduced as a criterion for assessment of acute trigeminal pain animal models as a behavioral method [13] [14]. This study was designed to evaluate the probable antinociceptive role of the acute and chronic oral administrations of pomegranate juice and seed extract on hypertonic saline-induced acute corneal pain perception in mice and compared it with analgesic opioid system to identify the mechanism
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
