Abstract
BackgroundPterodon pubescens Benth seeds are commercially available in the Brazilian medicinal plant street market. The crude alcoholic extracts of this plant are used in folk medicine as anti-inflammatory, analgesic, and anti-rheumatic preparations. The aim of this study was to evaluate the contribution of geranylgeraniol (C1) and 6α, 7β-dihydroxyvouacapan-17β-oate methyl ester (C2) isolated from Pterodon pubescens Benth. to the antinociceptive activity of the crude extract.ResultsCompounds C1 and C2 demonstrated activity against writhing with intraperitoneal (i.p.) and oral (p.o.) routes, capsaicin (i.p. and p.o.), glutamate (i.p.), and in the hot-plate (p.o.) tests, demonstrating their contribution to the antinociceptive activity of crude Pterodon pubescens Benth extracts. The observed activity of compounds C1 and C2 may be related to vanilloid receptors VR1, and/or glutamate peripheral receptors. In hot-plate model, the antinociceptive activity was maintained when naloxone chloride (opioid antagonist) was administered prior to treatment with compounds suggesting that C1 and C2 (p.o.) do not exert their antinociceptive effects in the hot-plate test via opioid receptors. The findings presented herein also suggest that compounds within the crude Pterodon pubescens Benth. extract may exert a synergistic interactive effect, since the crude extract (300 mg.kg-1) containing lower concentrations of compounds C1 (11.5%- 34.6 mg. kg-1) and C2 (1.5% - 4.7 mg.kg-1) gave statistically the same effect to the pure compounds when tested separately (C1 = C2 = 300 mg.kg-1) in writhing experimental model with p.o. administration. Further studies will be undertaken to establish more specifically the mechanisms of action for compounds C1 and C2. Possible synergistic interactions will be evaluated employing the Isobole method.ConclusionThese results allowed us to establish a relationship between the popular use of Pterodon pubescens seeds for pain relief and the activity of two major compounds isolated from this species which demonstrated antinociceptive activity. Various "in vivo" experimental models corroborate the folk use of this species for different pain and inflammation disorders.
Highlights
Pterodon pubescens Benth seeds are commercially available in the Brazilian medicinal plant street market
We report the antinociceptive activity of 6a, 7b-dihydroxyvouacapan-17b-oate methyl ester and geranylgeraniol isolated from Pterodon pubescens Benth. when evaluated in writhing, capsaicin, glutamate and hot-plate animal experimental models
Some authors have reported the antinociceptive activity of the crude extract and fractions obtained from P. pubescens and established a relationship with anti-inflammatory activity [10,12]
Summary
Pterodon pubescens Benth seeds are commercially available in the Brazilian medicinal plant street market. The crude alcoholic extracts of this plant are used in folk medicine as anti-inflammatory, analgesic, and anti-rheumatic preparations. The aim of this study was to evaluate the contribution of geranylgeraniol (C1) and 6a, 7b-dihydroxyvouacapan-17b-oate methyl ester (C2) isolated from Pterodon pubescens Benth. To the antinociceptive activity of the crude extract. (Leguminosae), known as sucupira, is widespread throughout the Brazilian states of Goiás, Minas Gerais and São Paulo. Sucupira seeds are commercially available in the Brazilian medicinal plant market. The crude alcoholic extracts of this plant are used in folk medicine previously reported to be associated with the antiinflammatory activity of these species [8]. We report the antinociceptive activity of 6a, 7b-dihydroxyvouacapan-17b-oate methyl ester and geranylgeraniol isolated from Pterodon pubescens Benth. We report the antinociceptive activity of 6a, 7b-dihydroxyvouacapan-17b-oate methyl ester and geranylgeraniol isolated from Pterodon pubescens Benth. when evaluated in writhing, capsaicin, glutamate and hot-plate animal experimental models
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have