Antimutagenic and Multi-Biological Activities of Smilax excelsa L. Fruit Extract

Abstract

In this study, antimutagenic, antimicrobial and antioxidant activities of Smilax excelsa L. fruit extract were evaluated. The antimicrobial effect was investigated by disk diffusion method and the antimutagenic effect was investigated by Ames/Salmonella/microsom test. The antioxidant properties of S. excelsa fruit samples were determined by investigating the total phenolic, flavonoid contents and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. It was observed that 200 mg plate-1 fruit extract was found to have a mutagenicity inhibition as 81% in the absence of S9 mixture and 67% in the presence of S9 mixture against Salmonella typhimurium TA1535. S. excelsa fruit extract produced an inhibition zone in the range of 11-16 cm against the tested microorganisms. Flavonoid and phenolic contents were found to be 0.7985±0.0124 mgQE 100 mL-1 and 11.9847±0.0041 mgGAE 100 mL-1 at the 200 mg mL-1 extract concentration, respectively. The DPPH removal rate was determined to be 55% at the 200 mg L-1 dose. As a result, it was observed that the S. excelsa fruit tissues exhibited a highly antimutagenic activity and has been determined as a potential natural antimicrobial and antioxidant source.