Abstract
BackgroundThree extracts were prepared from the leaves of Accacia salicina; ethyl acetate (EA), chloroform (Chl) and petroleum ether (PE) extracts and was designed to examine antimutagenic, antioxidant potenty and oxidative DNA damage protecting activity.MethodsAntioxidant activity of A. salicina extracts was determined by the ability of each extract to protect against plasmid DNA strand scission induced by hydroxyl radicals. An assay for the ability of these extracts to prevent mutations induced by various oxidants in Salmonella typhimurium TA102 and TA 104 strains was conducted. In addition, nonenzymatic methods were employed to evaluate anti-oxidative effects of tested extracts.ResultsThese extracts from leaf parts of A. salicina showed no mutagenicity either with or without the metabolic enzyme preparation (S9). The highest protections against methylmethanesulfonate induced mutagenicity were observed with all extracts and especially chloroform extract. This extract exhibited the highest inhibitiory level of the Ames response induced by the indirect mutagen 2- aminoanthracene. All extracts exhibited the highest ability to protect plasmid DNA against hydroxyl radicals induced DNA damages. The ethyl acetate (EA) and chloroform (Chl) extracts showed with high TEAC values radical of 0.95 and 0.81 mM respectively, against the ABTS.+.ConclusionThe present study revealed the antimutagenic and antioxidant potenty of plant extract from Accacia salicina leaves.
Highlights
Three extracts were prepared from the leaves of Accacia salicina; ethyl acetate (EA), chloroform (Chl) and petroleum ether (PE) extracts and was designed to examine antimutagenic, antioxidant potenty and oxidative DNA damage protecting activity
The present study was designed to examine antimutagen, antioxidant potenty and oxidative DNA damage protecting activity of plant extract from Accacia salicina leaves in relation to their total polyphenol, tannin, sterol and flavonoid content
Determination of Total Polyphenol, Flavonoid, tannins and sterols Contents The phytochemical study of A. salicina extracts showed the presence of various quantities of polyphenols, sterols, tannins and flavonoids (Table 2)
Summary
Three extracts were prepared from the leaves of Accacia salicina; ethyl acetate (EA), chloroform (Chl) and petroleum ether (PE) extracts and was designed to examine antimutagenic, antioxidant potenty and oxidative DNA damage protecting activity. Oxidative stress, caused by reactive oxygen species (ROS), is known to cause the oxidation of biomolecules, leading to cellular damage. The tissue injury caused by ROS may include DNA protein and lipids damage [2,3]. Antigenotoxic plant can counter or prevent the adverse effect caused by DNAdamaging chemicals [4]. The present study was designed to examine antimutagen, antioxidant potenty and oxidative DNA damage protecting activity of plant extract from Accacia salicina leaves in relation to their total polyphenol, tannin, sterol and flavonoid content
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