Abstract
Antimutagenic and DNA protective effect of an extract VinOserae from Vitis vinifera grapes on oxidative DNA damage was investigated. The extract’s ability to inhibit mutagenicity induced by tert-butyl hydroperoxide (t-BHP) and hydrogen peroxide (H2O2) was determined with Ames test using Salmonella typhimurium His? TA102 strain. Inhibition values of 44.2% and 67.0% were detected for t-BHP and H2O2, respectively. A protective ability of the extract against DNA strand scission induced by hydroxyl radicals was studied with plasmid pBluescript II SK(-). The analysis of DNA strand breaks in plasmid DNA showed a significant inhibition of DNA damage.
Highlights
Plants have long been considered as a valuable source of compounds beneficial for humans
Many compounds that are found in plants exhibit a biological activity such as antimicrobial, antioxidative, antiviral, antiinflammatory, antimutagenic and anticarcinogenic [1]-[5]
Detection of the antimutagenic activity of the VinOserae extract was carried out using the Ames test with Salmonella typhimurium His‒ TA102
Summary
Plants have long been considered as a valuable source of compounds beneficial for humans. Many compounds that are found in plants exhibit a biological activity such as antimicrobial, antioxidative, antiviral, antiinflammatory, antimutagenic and anticarcinogenic [1]-[5]. Polyphenolics in medicinal and fragrant plants, fruit and vegetables belong to such compounds. Their broad protective effect consists in a number of individual complementary actions contributing to the upbuilding and maintenance of homeostasis. These compounds can help prevent harmful consequences of oxidative damage to biomolecules due to their biological activity and their action play important roles in prevention of various diseases such as cancer or cardiovascular and neurodegenerative diseases [6]-[9]. The antioxidative activity of polyphenolic compounds is given by the ability to bind reactive forms of oxygen and transition metal ions that catalyze formation of free radicals and, to inhibit enzymes involved in formation of free radicals [10]-[14]
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