Abstract
Sandy tea is a traditional and popular drink in China. The aim of this study was to determine the antioxidant activity, antimutagenic activity of sandy tea and its in vitro anticancer effect on TCA8113 human tongue squamous carcinoma cells. The antioxidant effects were determined by measuring the 1,1‐diphenyl‐2‐picryhydrazyl (DPPH) free radical舉 and hydroxyl radical舉scavenging activity. The sandy tea extract demonstrated high antioxidant activity in the assays of DPPH and hydroxyl radical舉scavenging activity. At concentrations of 1.25 and 2.5 mg/plate, sandy tea exhibited anti‐mutagenicity with N‐methyl‐N'‐nitro‐N‐nitrosoguanidine (MNNG) in the Salmonella typhimurium TA100 strain. MTT assay and RT‐PCR assay were employed to check the anticancer effects on TCA8113 cancer cells. At the concentration of 200 µg/ml, sandy tea (81%) showed the best inhibitory effect on TCA8113 human tongue squamous carcinoma cells compared to the other concentrations. Compared with 100 µg/ml and 50 µg/ml of sandy tea extracts, 200 µg/ml of sandy tea induced apoptosis, up‐regulated mRNA expressions of Bax, caspase‐3 and caspase‐9, and down‐regulated Bcl‐2 expression. These results suggest that sandy tea possesses good antioxidant activity and anticancer effect on TCA8113 human tongue squamous carcinoma cells.
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