ANTIMOTILITY AND ANTISECRETORY RELATED ANTIDIARRHOEAL ACTIVITY OF THE ABELMOSCHUS MOSCHATUS MEDIK IN EXPERIMENTAL ANIMAL MODELS

Babitha S,Nageena Taj,Akshay Deepu Hg

doi:10.22159/ijpps.2018v10i5.24450

Abstract

Objective: This study was intended to evaluate the anti-diarrhoeal potential of Abelmoschus moschatus Medik (A. moschatus Medik) seeds and the possible mechanism therein involved by using different experimental models in albino Wistar rats.Methods: The hydroalcoholic seed extract of A. moschatus Medik. (HEAM) was orally administered at the doses of 150, 300 and 500 mg/kg respectively to the different groups in order to assess the effect of extract in castor oil induced diarrhea model in rats. In order to comprehend the mechanism involved in its anti-diarrhoeal potential, the extract was further investigated for its effect on gastrointestinal motility using charcoal meal test and antisecretory action by castor oil induced intestinal enter pooling where, atropine sulphate (5 mg/kg) and loperamide (2 mg/kg) were used as reference standards respectively.Results: The HEAM exhibited significant (p<0.05, p<0.001) and dose-dependent anti-diarrhoeal effect by decreasing the mean number of fecal droppings produced upon castor oil administration as compared to the normal control. The effect of the extract at 500 mg/kg was near to that of loperamide (2 mg/kg). The extract (300 mg/kg) showed antimotility action by significantly (p<0.05, p<0.001) attenuating the charcoal meal transit in the intestine as compared to negative control animals. Further, the extract showed significant (p<0.05, p<0.001) inhibition in the accumulation of intestinal fluid due to castor oil.Conclusion: The HEAM exhibited significant anti-diarrhoeal action that could be presumably related to its observed antimotility and antisecretory activities. This study justifies the usage of A. moschatus Medik. as an anti-diarrhoeal agent in traditional practices of medicine.

Highlights

Diarrhoel diseases are the most important causes of illness and mortality throughout the globe
In the system of traditional medicine, A. moschatus Medik is being employed in the management of diarrhoea, antispasmodic, GI complaints by traditional healers at a lower cost
It can be presumed that the mechanism of anti-diarrhoeal effect of the HEAM was intervened through an antisecretory action

Summary

Introduction

Diarrhoel diseases are the most important causes of illness and mortality throughout the globe. Inadequate sanitation and poor hygiene are the causes for about 88% of diarrhoel-related deaths [1]. A critical global health problem, in developing nations, accounts for over 5-8 million mortalities in children below 5 y of age, every year [2]. Plants have been vital resources for new agents. Several species of plants have been evaluated for substances with therapeutic effect [3]. It is well known that herbal medicines are fairly safe, inexpensive and accessible to the layman, as compared to that of synthetic drugs. The herbal remedies or products obtained from the natural origin are known to possess synergistic and/or side effects counteracting potentials and offer their pharmacological actions mediated normally through several pathways [1]

Methods

Results

Discussion

Conclusion