Antimony(III) induces fibroblast-like phenotype, profibrotic factors and reactive oxygen species in mouse renal cells

Abstract

Environmental contextAntimony is a metalloid occurring at overall low concentrations in the Earth’s crust. Owing to anthropogenic activities, however, antimony can be found at elevated and detrimental levels in some environments. We report eco-toxicological effects of antimony in biological models, results from which can help predict antimony’s ecological and environmental impact. AbstractThe aim of this work was to evaluate the effect of SbIII on cell integrity, expression of profibrotic factors and reactive oxygen species (ROS) in mouse cortical collecting duct cells (M-1 cell line). M-1 cells were incubated with SbIII for 24h. Cell morphology and RNA expression level (connecting tissue growth factor, CTGF), α-SMOOTH MUSCLE ACTIN (α-SMA) and collagen I were analysed. The total Sb content according to each cell compartment was determined and ROS production was measured. Eighty percent of the total Sb was detected in the extracellular medium. A significant increase in ROS production and CTGF protein expression were observed at 100μgL−1 SbIII. M-1 cells showed a non-classic epithelial cell shape at 100μgL−1 and a reduction in the collecting duct-specific marker aquaporin-2. At 100μgL−1, the number of collagen I-positive cells increased. At 300μgL−1, a gross cell nuclear rupture was observed. These results demonstrate that an SbIII concentration of 100μgL−1 is able to promote the induction of CTGF and collagen I along with the induction of ROS, which suggests a cytotoxicity of SbIII in M-1 kidney collecting duct cells.