Antimicrobial susceptibility, virulence genes and genomic characterization of Trueperella pyogenes isolated from abscesses in dairy cattle

Abstract

Trueperella pyogenes is an opportunistic animal pathogen mainly associated with various suppurative infections in wild and domestic animals. Limited studies have investigated the pathogenesis of diseases caused by this pathogen. The main objective of the current study was to investigate the prevalence, phenotypic properties, virulence genotypes, antimicrobial susceptibility and genetic characterization of T. pyogenes isolated from abscess lesions in different tissues of on-farm dairy cattle. The study was performed on 150 postpartum cattle with clinical abscess symptoms on 22 farms around Tehran, Iran. Classical and disk diffusion methods are used for phenotypic characterization and antibiotic susceptibility. Detection of virulence factor encoding genes and genomic characterization of the isolates also are carried out by conventional PCR and BOX-PCR assays, respectively. Sixty-eight T. pyogenes strains (45.3%) were isolated, 12 were identified as pure cultures and the other 56 strains were isolated from mixed cultures. Seven distinct biotypes were identified among the T. pyogenes isolates. The isolates were mostly resistance to trimethoprim-sulfamethoxazole (70.6%), erythromycin (36.7%), tetracycline (26.5%) and tylosin (23.5%) antibiotics. Also, the genes plo, nanH, nanP and fimA were detected in all isolates. Forty-two isolates (61.7%) carried all virulence factor genes detected in this study. Three isolates only carried plo, nanH, nanP and fimA genes were identified as the least frequent genotype. All sixty-eight isolates and the reference strain were categorized into seven main clusters (A-G). A strong association was observed between virulence factor encoding genes, pathogenicity and biochemical biotypes in some specific clonal relationships.