Abstract
Antimicrobial susceptibility testing (AST) is a widely used method to assess the antimicrobial action of an sample or a pure molecule, in order to be used in applications for public health, the economy, and the environment. Currently, there are multiple AST methods available to determine an antibiotic-resistant bacterium's susceptibility. Several methods also are employed to assess how various antibacterial drugs affect bacteria. The CLSI publications provide guidance for the most important drugs to analyze and report on in relation to such organisms, as well as value ranges to ensure precise & reproducible findings. The responsibilities are to ensure breakpoints to evaluate MICs or disc diffusion zone measurements. The disc diffusion technique is easy, useful, and works very well. The agar well diffusion methodology of Aspergillum essential oil is applied to examine the antibacterial activity of plants, bacterial extracts, or pure substances, or to examine the competition between microorganisms.
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