Abstract
This study was conducted to determine antibiotic susceptibility of Flavobacterium psychrophilum isolated in diseased rainbow trout (Oncorhynchus mykiss) from two hatcheries in the Mediterranean region of Turkey. The samples were taken from the kidney and spleen for bacteriology from rainbow trout juveniles (weight 0.7-1 g) in April and isolates obtained on tryptone yeast extract salts (TYES) agar medium at 15°C for 72 hours. Fourteen isolates were identified as F. psychrophilum using biochemical, physiological, and morphological characteristics and API ZYM test. Antibiotic sensitivities of the isolates were determined by the disc diffusion method on TYES agar, and the results were evaluated according to CLSI standards. Only one isolate from each hatchery was used for the antibiogram test. Both isolates were sensitive to amoxicillin/clavulanic acid 2:1, penicillin, nitrofurantoin, florfenicol, amoxicillin, clindamycin, chloramphenicol, ampicillin, doxycycline, streptomycin, and erythromycin; resistant to sulfadiazine, sulfamethoxazole/trimethoprim, oxolinic acid, vancomycin, trimethoprim/sulfadiazine, gentamicin, kanamycin, nalidixic acid, oxacillin, enrofloxacin, flumequine, tobramycin. The results show that isolating F. psychrophilum and performing the antimicrobial susceptibility testing is important issue in disease control.
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