Abstract
BackgroundCampylobacter is a leading cause of foodborne gasteroenteritis worldwide. Antimicrobial susceptibility testing for Campylobacter spp. is not routinely performed by most clinical laboratories. However, the emergence of resistant isolates strengthens the importance of antimicrobial susceptibility testing and the critical need for epidemiologic surveillance. The aim of this study was to compare the efficacy of Etest and Sensititre kit (a broth microdilution method) as methods for susceptibility tests and the critical need for epidemiologic surveillance. The aim of this study was to compare the efficacy of Etest and Sensititre kit (a broth microdilution method) as methods for susceptibility testing of Campylobacter spp. to tetracycline, erythromycin, and ciprofloxacin.MethodsSixty-six Campylobacter isolates were collected from feces samples and subjected to susceptibility testing by Etest and Sensititre, a broth microdilution kit for tetracycline, erythromycin, and ciprofloxacin. Minimal inhibitory concentration (MIC) results of each method were determined and compared.ResultsSimilar MIC interpretations for tetracycline, erythromycin, and ciprofloxacin were found in 97%, 98.5%, and 100% of the isolates, respectively, indicating a good level of agreement between Etest and Sensititre (p < 0.0001); additionally, the correlation between the two methods was highly significant for the three tested antibiotics (p < 0.0001).ConclusionsBoth the broth microdilution and the Etest are reliable and convenient methods for testing antimicrobial susceptibility of Campylobacter spp. The Sensititre kit has the advantages of high availability and the automation.
Highlights
Campylobacter is a leading cause of foodborne gasteroenteritis worldwide
Sixty-six Campylobacter isolates were collected in the current study; of these, 50 Campylobacter jejuni and 16 Campylobacter coli were identified
All of which were C. jejuni, were interpreted as sensitive according to Etest and resistant according to Sensititre
Summary
Campylobacter is a leading cause of foodborne gasteroenteritis worldwide. The emergence of resistant isolates strengthens the importance of antimicrobial susceptibility testing and the critical need for epidemiologic surveillance. The aim of this study was to compare the efficacy of Etest and Sensititre kit (a broth microdilution method) as methods for susceptibility tests and the critical need for epidemiologic surveillance. The aim of this study was to compare the efficacy of Etest and Sensititre kit (a broth microdilution method) as methods for susceptibility testing of Campylobacter spp. to tetracycline, erythromycin, and ciprofloxacin. Campylobacter is a leading cause of foodborne gastroenteritis worldwide [1]. In Israel, which is characterized by a high Campylobacter morbidity, there is a requirement to report Campylobacter infections to the Ministry of Health [7]. While in 2010 the incidence of Campylobacter infections in the USA was 12.3 per 100,000 population [8], in Israel, the incidence has increased 2.93-fold since 1999 (from 31.04 to 90.99 cases per 100,000 population) [7]
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